FIELD: chemistry.
SUBSTANCE: invention deals with method for detecting serological affinity of microbes, which have common determinants in composition of lipopolysaccharides. Method includes obtaining specific antibodies against lipopolysaccharides of "model strain", serological affinity to which it is necessary to determine. Method includes: growing cultures of analysed strains of bacteria of genus Azospirillum and addition of antibodies in "working" concentration to bacterial suspensions with further preparation of specimens for light microscopy, assessment of motility of bacterial of genus Azospirillum, detection of bacteria, which have common determinants in composition of polysaccharides, by reduction of motility of bacterial if genus Azospirillum in presence of antibodies against "model strain" lipopolysaccharides. As "working" concentration of antibodies, used is doubled concentration of antibodies, in presence of which all cells of homologous model strain of bacteria of genus Azospirillum are immobile.
EFFECT: claimed invention makes it possible to reduce time for detection of serologically affined strains of bacteria of genus Azospirillum directly in liquid bacterial cultures.
1 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING CELL SURFACE PROTEINS | 2017 |
|
RU2670001C1 |
| COMPOSITION FOR TRASPORTING ACTIVE SUBSTANCES TO WHEAT ROOTS | 2007 |
|
RU2347366C2 |
| CULTIVATED HYBRID CELL STRAIN OF Mus musculus L ANIMALS - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO O-ANTIGENE OF CHOLERA VIBRIO SEROGROUP O1 | 2010 |
|
RU2425874C1 |
| METHOD FOR INCREASING GERMINATING CAPACITY OF WHEAT SEEDS INFECTED WITH TURTLE BUGS | 2006 |
|
RU2314692C1 |
| METHOD OF BIODEGRADATION OF MALACHITE GREEN (OPTIONS) | 2017 |
|
RU2676153C2 |
| STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS MUS MUSCULUS L - PRODUCER OF MONOCLONAL ANTIBODIES TO BACTERIA OF BRUCELLA GENUS | 0 |
|
SU1752764A1 |
| CULTIVATED HYBRID CELL STRAIN OF Mus Musculus L ANIMALS - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO LPS OF CHOLERA VIBRIO 0139 SEROGROUP | 2010 |
|
RU2425875C1 |
| METHOD FOR PREPARING LIPOPOLYSACCHARIDES | 2002 |
|
RU2237719C2 |
| STRAIN OF CULTURED ANIMAL HYBRID CELLS MUS. MUSCULUS - PRODUCER OF MONOCLONAL ANTIBODIES TO THE MEMBRANE PROTEIN COMMON TO TYPICAL AND ATYPICAL SEROGROUP 01 CHOLERA VIBRIOS | 2022 |
|
RU2785463C1 |
| PROTEIN NMB0928 AND ITS APPLICATION IN PHARMACEUTICAL COMPOSITIONS | 2004 |
|
RU2335505C2 |
