K5 HEPAROSAN FERMENTATION AND PURIFICATION Russian patent published in 2015 - IPC C12P19/04 C08B37/10 C12N1/20 C12R1/19 

FIELD: chemistry.

SUBSTANCE: method of producing substantially pure heparosan from E.coli K5, comprises culturing E.coli K5 in a medium with glucose as the primary carbon source, binding heparosan to a solid-phase carrier with subsequent elution and precipitating heparosan from the eluate. Said culturing includes batch culturing phase and a batch fed culturing phase. The medium used at the batch culturing step contains (per litre) about 20 g glucose, 10-300 mg thiamine, about 13.5 g KH₂PO₄, about 4.0 g (NH₄)₂HPO₄, about 1.4 g MgSO₄·7H₂O, about 1.7 g citric acid, and about 10.0 ml solution of trace elements, the solution of trace elements substantially consists of (per 1 l 5M HCl) 10.0 g FeSO₄·7H₂O, 2.0 g CaCl₂, 2.2 g ZnSO·7H₂O, 0.5 g MnSO₄·4H₂O, 1.0 g CuSO₄·5H₂O, 0.1 g (NH₄)₆Mo₇O₂₄·4H₂O and 0.02 g Na₂B₄O₇·10H₂O. The feed medium used at the batch fed culturing step contains (per litre): 250-1000 g glucose, 20 g MgSO₄, 0.15-0.5 g thiamine and, optionally, 47 g KH₂PO₄. Oxygen is supplied by bubbling air with or without additional supply of oxygen.

EFFECT: method enables to obtain at least 90% pure heparosan.

14 cl, 12 dwg, 1 tbl, 3 ex