METHOD OF OBTAINING PEGYLATED OLIGONUCLEOTIDES Russian patent published in 2015 - IPC A61K48/00 A61K47/48 A61K47/30 C07H21/00 

FIELD: chemistry.

SUBSTANCE: claimed invention relates to method of obtaining pegylated oligonucleotide, which can be used for obtaining biologically active substances. Method includes: (a) synthesis of non-pegylated oligonucleotide on solid carrier, (b) cleavage of non-pegylated oligonucleotide from solid carrier and removal of protective groups from oligonucleotide, (c) desalination of non-pegylated oligonucleotide with application of ultrafiltration, (d) pegylation of non-pegylated oligonucleotide to obtain pegylated oligonucleotide, (e) purification of pegylated oligonucleotide with application of anion-exchange HPLC, (f) desalination and additional purification of pegylated oligonucleotide with application of ultrafiltering membrane with threshold of molecular weight from 10 kDa to 30 kDa, where ion-exchange purification of non-pegylated oligonucleotide is not realised between stages (b) and (c).

EFFECT: claimed method is characterised by improved efficiency.

10 cl, 10 dwg, 11 tbl, 2 ex