FIELD: chemistry.
SUBSTANCE: invention relates to a method of conjugation of polysialic acid (PSA) or modified PSA (mPSA) with an oxidised carbohydrate part of glycoprotein, different from the blood clotting protein, which contains a carbohydrate group, by contact of the oxidised carbohydrate part with PSA or mPSA, where the said PSA or mPSA contains an aminooxygroup and an oxime bond is formed between the oxidised carbohydrate part and the aminooxygroup on PSA or mPSA.
EFFECT: method makes it possible to obtain high conjugate output, high preservation of the conjugated glycoprotein activity in comparison with the non-conjugated protein and high conjugation effectiveness.
14 cl, 2 dwg, 27 ex
| Title | Year | Author | Number |
|---|---|---|---|
| GLYCOPOLYSIALYLATION OF PROTEINS THAT ARE NOT THE PROTEINS OF THE BLOOD CLOTTING | 2014 |
|
RU2662807C2 |
| CONJUGATES OF BLOOD COAGULATION PROTEINS | 2010 |
|
RU2595442C2 |
| BLOOD COAGULATION PROTEIN CONJUGATES | 2010 |
|
RU2744370C2 |
| ALDEHYDE DERIVATIVES OF SIALIC ACID, METHODS OF THEIR OBTAINMENT, CONJUGATES OF ALDEHYDE DERIVATIVES OF SIALIC ACID, AND PHARMACEUTICAL COMPOSITION BASED ON THEM | 2004 |
|
RU2333223C2 |
| N-TAILED POLYSIALYLATION | 2007 |
|
RU2432175C2 |
| POLYSIALIC ACID DERIVATIVES | 2004 |
|
RU2327703C2 |
| METHOD OF REDUCING CONTENT OF ENDOTOXIN IN SAMPLE CONTAINING POLYSIALIC ACID AND ENDOTOXIN | 2008 |
|
RU2473567C2 |
| DRUG PREPARATION AND METHOD FOR IMPROVING RHEOLOGICAL PROPERTIES OF SPUTUM AND PULMONARY ADMINISTRATION OF THIS PREPARATION | 2009 |
|
RU2522846C2 |
| METHOD FOR OBTAINING A HYBRID PROTEIN CONTAINING A FUSED PROTEIN ANALOGUE OF AN INTERFERON GAMMA CONJUGATED WITH OLIGOSACCHARIDE | 2016 |
|
RU2656140C2 |
| SITE-SPECIFIC ANTIBODY-DRUG CONJUGATION BY MEANS OF GLYCOENGINEERING | 2014 |
|
RU2711935C2 |
