FIELD: biotechnologies.
SUBSTANCE: enzyme serine fungus protease with amino-acid sequence of mature enzyme is described which at least is 86% identical to the amino-acid sequence of mature Fe_RF6318 enzyme presented in the description. The following is described: the released nucleinic acid coding the named enzyme; the recombinant expression vector containing the named nucleinic acid functionally joined with signs; and the host cell for obtaining of enzyme of serine protease according to the present invention containing the named recombinant expression vector. The method of obtaining of enzyme or fermental preparation containing the named enzyme of serine protease is offered, also the method includes the stage of cultivation of the host cell according to the present invention and either the stage of restoration of protease from the cell, or the stage of release of the cell from the cultural medium and obtaining the supernatant containing protease. The fermental preparation containing the serine fungus protease described in the invention, obtained by the named method, is offered.
EFFECT: invention allows to expand the arsenal of proteolytic enzymes.
38 cl, 17 dwg, 6 tbl, 14 ex
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Authors
Dates
2015-10-27—Published
2010-04-30—Filed