FIELD: biotechnology.
SUBSTANCE: method for production of a protease protein by a bacterium of the Bacillus genus by the first expression construct introduction therein that encodes the target protease protein and the second expression construct that encodes an auxiliary protease with an amino acid sequence of SEQ ID NO: 1 different from the target protein and further expression of the said proteases in the said microorganism. A Bacillus genus microorganism is presented to obtain protease, which includes the first expression construct encoding the specified target protease and the second expression construct encoding an auxiliary protease different from the target protein with an amino acid sequence of SEQ ID NO: 1.
EFFECT: group of inventions allows to increase the yield of target protease protein at microbiological fermentation.
8 cl, 4 dwg, 2 ex
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Authors
Dates
2018-01-24—Published
2012-04-05—Filed