METHOD OF DETECTING LIPASE-PRODUCING MICROORGANISMS IN CONFECTIONARY PRODUCTS Russian patent published in 2015 - IPC C12Q1/04 C12N1/20 C12N9/20 

FIELD: chemistry.

SUBSTANCE: invention relates to microbiology and can be applied for detection of lipase-producing microorganisms in confectionery products. Method of detecting lipase-producing microorganisms in confectionary products includes sampling, preparation of initial dilution of sample from sample and a series of tenfold dilutions with the following inoculation in two parallel Petri dishes. Inoculations are poured with nutrient medium, which contains peptone, sodium chloride, calcium chloride, agar, fatty emulsion and alcohol blue dye in specified ratio of components and are incubated at temperature 30±1°C for 48±3 h in aerobic conditions. After incubation of inoculations performed is calculation of colonies of typical microorganisms, which produce lipolytic enzymes, to which colonies, which have blue coloration around and/or under them, and/or white dimness of medium, are referred to.

EFFECT: increased method efficiency.

1 dwg