BIOLOGICAL METHOD OF ANALYSIS FOR CONTROL OF QUALITY OF NUTRACEUTICALS AND MEDICATIONS Russian patent published in 2015 - IPC C12Q1/68 G01N33/68 G01N33/92 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of biotechnology. Method for detection of substance, which mediates positive regulation of biological marker gene transcription, or determination, if substance mediates positive regulation of biological marker gene transcription, consists of the following stages: provision of first analysis system, which includes mRNA sequence, which contains region, which codes first reporter protein, functionally bound with promoter of first biological marker; provision of second analysis system, which includes second mRNA sequence, which contains region, which codes second report protein, functionally bound with promoter of second biological marker; realisation of contact of said second analysis system with substance; determination of levels of transcription of said first and second mRNA sequences. Level of transcription of second mRNA sequence is higher than level of transcription of first mRNA sequence, if said substance mediates positive regulation of biological marker gene transcription. Genes of first biological marker and second biological marker are selected from the group, which consists of proapoptotic protein, homological to C/EBP (CHOP), binding of chaperonic protein of endoplasmatic reticulum (BiP), factor of transcription activation 4 (ATF-4) protein 1, which binds X-box (Xbp-1) and synthase of amino acids.

EFFECT: increase of method efficiency.

14 cl, 1 dwg, 4 ex