TRANS-REPLICATING RNA Russian patent published in 2021 - IPC C12N15/86 C12N15/40 C12N5/10 A61K48/00 

FIELD: biotechnology.

SUBSTANCE: group of inventions is described, including a system for producing a protein, a pharmaceutical composition for expression of alphavirus replicase and/or replicon, a method for producing a protein in a cell, an eucaryotic host cell for producing a protein, and a method for producing a protein in a subject. In one of the embodiments, the system contains RNA structure for expression of alphavirus replicase and RNA replicon, which can be replicated by replicase in trains, wherein RNA structure contains 5' UTR, an open reading frame, encoding replicase, and 3' UTR, where RNA structure contains 5’-cap for replicase transmission, and RNA replicon contains 5' replicative sequence of alphavirus recognition, an open reading frame encoding the protein of interest, and 3' replicative sequence of alphavirus recognition.

EFFECT: obtaining trans-replicating RNA for producing a protein.

15 cl, 8 dwg, 7 ex