RNA REPLICON FOR UNIVERSAL AND EFFECTIVE GENE EXPRESSION Russian patent published in 2021 - IPC C12N15/86 C12N15/85 C12N5/10 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. A group of inventions is described, including an RNA replicon for protein expression, a trans-replication system for protein expression, including the above RNA replicon, DNA for use as a template for transcription in vivo or in vitro, a method for producing a protein of interest in a cell (variants), a host cell for producing a protein, a method for producing a protein of interest from a subject (variants). In one embodiment, the RNA replicon comprises a modified 5 'replication recognition sequence of an alpha-virus, wherein the 5' replication recognition sequence is modified by removing all translation initiation codons as compared to the native alpha-virus 5 'replication recognition sequence, wherein the alpha-virus is a Semliki Forest virus or a Sindbis virus.

EFFECT: invention expands the arsenal of tools for protein replication.

23 cl, 9 dwg, 8 ex