METHOD FOR DETERMINATION OF n-3 POLYUNSATURATED FATTY ACID ACTIVITY IN FISH OIL SAMPLE Russian patent published in 2018 - IPC C12Q1/68 G01N33/68 G01N33/92 

FIELD: biotechnology.

SUBSTANCE: in vitro method for determination of the presence of n-3 polyunsaturated fatty acid (PUFA) activity in a fish oil sample is provided. The first test system comprising the first mRNA sequence encoded by the first biomarker gene that has the first reporter protein coding region operably linked to the first marker promoter, is provided. The first test system is brought into contact with a standard substance or control substance. The second test system comprising the second mRNA sequence encoded by the second biomarker gene that has the second reporter protein coding region operably linked to the second marker promoter, is provided. The second test system is brought into contact with a fish oil sample. The first and the second marker genes are selected from the group consisting of nucleic acid sequences that encode a proapoptotic protein homologous to C/EBP (CHOP), ER protein, chaperone binding (BiP), transcription activation factor 4 (ATF-4), and protein I, connecting the X-box (Xbp-1). The transcription levels of the first and second mRNA sequences are determined. The fish oil sample is determined as having PUFA activity if the sample mediates positive regulation of marker gene transcription and the transcription level of the second mRNA sequence is higher than the transcription level of the first mRNA sequence.

EFFECT: invention allows to examine food, nutraceuticals and medicines in terms of n-3 PUFA activity presence.

13 cl, 1 dwg, 4 ex