NON-REPLICATIVE TRANSDUCTION PARTICLES AND TRANSDUCTION PARTICLE BASED REPORTER SYSTEMS Russian patent published in 2018 - IPC A61K35/76 C12Q1/04 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry and virology and relates to methods and systems for packaging reporter nucleic acid molecules in non-replicative transduction particles for use as reporter molecules. Bacterial cell packaging system for packaging the reporter nucleic acid molecule into non-replicative contains a lysogenised bacteriophage genome that does not contain the first bacteriophage gene encoding the first sequence of the packaging initiation site, wherein the removal of said first bacteriophage gene prevents the packaging of the bacteriophage nucleic acid molecule into said non-replicative transduction particle; and a plasmid with a reporter nucleic acid molecule containing a reporter gene containing a second bacteriophage gene, wherein said second bacteriophage gene encodes a second sequence of the packaging initiation site and facilitates packaging of a copy of said plasmid with a reporter nucleic acid molecule into said non-replicative transduction particle, wherein said second bacteriophage gene is capable of expressing a protein that is encoded by said second gene, wherein said copy of said plasmid with a reporter nucleic acid molecule forms a replicon that can be packaged into said non-replicative transduction particle, said bacteriophage being selected from the bacteriophage P1 Enterobacteriaceae, bacteriophage ϕ80α or bacteriophage ϕ11 S. aureus.

EFFECT: invention makes it possible to obtain non-replicative transduction particles that do not suffer from harmful effects of the lytic functions of the virus.

136 cl, 35 dwg, 13 tbl, 10 ex