METHOD OF SEPARATING AND PURIFYING TARGET PROTEIN WITHOUT ADMIXTURE OF PRION PROTEIN PrP Russian patent published in 2013 - IPC C07K14/47 C07K1/18 C07K1/20 C07K1/16 A61L2/00 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to field of biochemistry. Claimed is method of separation and purification of target protein by chromatography, in which chromatography removes or reduces content of prions (PrP^Sc). Realised is interaction of potentially PrP^sc-contaminated sample, which contains target protein, with combined chromatographic material, which contains ligand. Ligand is selected from: positively charged N-benzyl-N-methylethanolamine ligand; negatively charged ligand of 2-(benzoylamino)butamoic acid; phenylpropyl ligand; N-hexyl ligand; 4-mercaptoethylpyridine ligand; ligand of 3-[{3-methyl-5-((tetra hydrofuran-2-ylmethyl)amino)-phenyl}amino]benzoic acid. Than buffer conditions in chromatographic conditions are created, such as, target protein binds with combined chromatographic material, and PrP^sc does not bind with combined chromatographic material. Then target protein is eluted. Also claimed is fraction of pharmaceutically applicable target protein with reduced content of prion protein.

EFFECT: invention makes it possible to obtain fraction of target protein with reduced content of PrP^sc from >1 to 4 lg(10) by application of single chromatographic resin.

9 cl, 3 tbl, 2 ex