METHOD OF AFFINITY CHROMATOGRAPHY TO OBTAIN PURIFIED PROTEIN Russian patent published in 2017 - IPC C07K1/22 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology. Washing of affinity chromatography matrix is made with flushing solution containing arginine or derivative of arginine, with pH value of approximately 8.5–9.5, where said washing is carried out without non-buffered salt. Derivative of arginine is selected from group consisting of acetylarginine, agmatine, arginine acid, N-alpha-butyroyl-arginine, N-alpha-pivaloyl-arginine, 3-guanidinopropionic acid, 4-guanidinebutyric acid, L-2-amino-3-guanidinopropionic acid hydrochloride, N_ωnitro-L-arginine benzyl ester p-toluenesulfonate salt and homoarginine. Invention increases concentration of target protein in pool and leads to 3-multiple reduction of content of host cell proteins, 4-multiple reduction of content of low-molecular compounds and to decrease of level of formation of aggregates.

EFFECT: method of producing purified protein of interest id disclosed using AC matrix, with which protein binds.

11 cl, 9 tbl, 3 ex