FIELD: biotechnology.
SUBSTANCE: invention relates, in particular, to methods for isolation of bacterial lipopolysaccharides and can be used for preparing chemical vaccines, immunomodulating agents and for construction of diagnostic test-systems. Extraction of lipopolysaccharides is carried out with buffer solution containing EDTA in the concentration 0.01-0.05 mmole per 1 g of wetted cells, 0.1 mmole of phenylmethylsulfonyl chloride and 1% of Triton X-100. Prepared extract is separated from microorganisms by centrifugation and subjected for treatment with enzyme proteinase K. Invention provides significant simplifying method for preparing lipopolysaccharides and to improve quality of the end preparation.
EFFECT: improved preparing method.
1 dwg, 3 ex
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