FIELD: biotechnology.
SUBSTANCE: method for producing surface proteins of gram-negative bacteria and plant cell culture. Method includes preliminary washing of TBS or PBS cells with buffer, treatment with 0.1–0.2 % solution of sodium deoxycholate for 5–10 minutes, filtering, precipitating the extracted surface proteins with a solution of trichloroacetic acid followed by precipitation, washing the precipitate with acetone, incubating 60–300 minutes at -18 °C, precipitation of surface proteins.
EFFECT: invention provides increased yield of the desired product.
5 cl, 4 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING BACTERIA OF GENUS AZOSPIRILLUM, WHICH HAVE COMMON ANTIGENIC DETERMINANTS IN COMPOSITION OF LIPOPOLYSACCHARIDES | 2013 |
|
RU2572350C2 |
| METHOD FOR PREPARING LIPOPOLYSACCHARIDES | 2002 |
|
RU2237719C2 |
| METHOD FOR PREPARING MEASLES VIRUS ANTIGEN FRACTION | 2000 |
|
RU2205022C2 |
| COMPOSITION BASED ON PEAS PROTEIN HYDROLYZATE FOR INCREASING SPECIFIC PRODUCER CELL LINES PRODUCTIVITY AND METHOD FOR PRODUCTION THEREOF | 2019 |
|
RU2741088C1 |
| COMPOSITION BASED ON HYDROLYZATE OF SUNFLOWER PROTEIN FOR INCREASING SPECIFIC PRODUCTIVITY OF PRODUCER CELL LINES AND METHOD FOR PRODUCTION THEREOF | 2019 |
|
RU2741086C1 |
| METHOD FOR INCREASING GERMINATING CAPACITY OF WHEAT SEEDS INFECTED WITH TURTLE BUGS | 2006 |
|
RU2314692C1 |
| METHOD OF OBTAINING PREPARATIONS OF SUB-CELL FRACTIONS OF TULAREMIA MICROBE | 2018 |
|
RU2680598C1 |
| RECOMBINANT pBM PLASMID AND METHOD OF OBTAINING NON-MARKER TRANSGENIC PLANTS, CAPABLE OF SYNTHESISING DESIRED PRODUCTS, USING SAID PLASMID | 2009 |
|
RU2410433C1 |
| ANTIGENIC PEPTIDES, METHOD OF DETECTION OF PARTICLES SECRETED BY TUMORS | 1993 |
|
RU2141969C1 |
| PROTEIN, METHOD OF PROTEIN PREPARING (VARIANTS), PHARMACEUTICAL COMPOSITION | 1992 |
|
RU2124024C1 |
