FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to molecular and medical biology, and particularly to genetically modified proteins. What is presented is Stx2[E167Q/R170H] protein, which carries point substitutions of two amino acid residues in a catalytic site region of Stx2A sub-unit, responsible for protonation of adenine of A4324 residue of 28S RNA of eucariotic ribosomes, namely a point substitution of [E167Q] glutamic acid residue in position 167 by a glutamine residue and a point substitution of [R170H] arginine residue in position 170 by a histidine residue. The produced protein has more than 106 times lower cytotoxicity and high immunogenicity.
EFFECT: invention can be applicable for animal immunisation, protecting against an action of wild-type Stx2 over-lethal doses, as well as a vaccine for immunisation against certain human and animal's intestinal infections.
4 dwg, 5 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| DNA SEQUENCE OF APTAMERS BINDING SHIGA-TOXIN OF TYPE 2 | 2014 |
|
RU2566552C1 |
| HUMANISED ANTIBODY OR ITS ANTIGEN-BINDING FRAGMENT (FAB) AGAINST FIRST AND/OR SECOND TYPE SHIGA LIKE TOXINS (EMBODIMENTS), COMPOSITION FOR TREATING ENTEROHAEMORRHAGIC ESCHERICHIA COLI TOXIC CONDITIONS, CONTAINING SAID ANTIBODIES AND/OR FAB | 2019 |
|
RU2732155C1 |
| ANTIGEN AND PROTEIN TOXIC CODING NUCLEOTIDE SEQUENCE CARRIER MICROORGANISM | 2007 |
|
RU2447145C2 |
| STRAIN OF HYBRID CULTURED CELLS MUS MUSCULUS 365E11 PRODUCING MONOCLONAL ANTIBODIES TO SHIGA-LIKE TOXIN TYPE II | 2020 |
|
RU2768838C1 |
| STRAIN OF ESCHERICHIA COLI SEROVAR O26:H11 BACTERIA, PRODUCER OF A SHIGA-LIKE TOXIN, DEPOSITED IN THE STATE COLLECTION OF PATHOGENIC MICROORGANISMS AND CELL CULTURES "SCPM-OBOLENSK" UNDER NUMBER B-8034 | 2020 |
|
RU2756980C1 |
| APPLICATION OF MODIFIED HOLLOW FIBROUS MATERIALS FOR REMOVAL OF EXOTOXINS, PRODUCED BY Escherichia coli, FROM LIQUIDS, MAINLY FROM BLOOD AND PLASMA, AS WELL AS THEIR APPLICATION FOR TREATMENT OF ACCOMPANYING DISEASES | 2012 |
|
RU2573492C2 |
| STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF SHIGA-LIKE TOXIN 2 (STX2) | 2023 |
|
RU2816500C1 |
| APPLICATION OF BOVINE LACTOFERRIN FOR PREPARING MEDICINAL AGENT FOR BACTERIA GROWTH INHIBITION | 2006 |
|
RU2399380C2 |
| METHOD FOR PREPARING NONTOXIC ANTI-ANTHRAX VACCINE | 2002 |
|
RU2287581C2 |
| RECOMBINANT PROTEIN FOR CHOLERA IMMUNIZATION | 2019 |
|
RU2723705C1 |
