METHOD OF INDUCING TARGETED DIFFERENTIATION OF MULTIPOTENT PROGENITOR CELLS OF PANCREAS IN INSULIN-PRODUCING BETA CELLS DURING DIABETES Russian patent published in 2016 - IPC C12N5/02 C12N5/71 C12N5/95 

FIELD: biotechnologies.

SUBSTANCE: invention relates to biotechnology, specifically to cell technologies and can be used in regenerative medicine. Suspension of non-adhesive mononuclear cells from tissues of pancreas during diabetes, in obtained suspension is estimated amount of multipotent progenitor cell count oligopotent progenitor cells and insulin-producing β-cells. Then for 30 days in presence of growth factors: 4 ng/ml of epidermal growth factor and 10 ng/ml of hepatocyte growth factor, extended cell mass multipotent progenitor cells. Upon completion of cycle of cultivation of induced differentiation of obtained in vitro multipotent progenitor CD45^- TER119^-, c-Kit^-, obtained 1^- cells in βcells glucagon-like peptide GLP-1 in concentration of 6.7 mcl/ml.

EFFECT: invention enables to efficiently induce differentiation of multipotent progenitor cells of pancreas in insulin-producing β-cells in type I diabetes mellitus.

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