FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, namely to production of a population of pancreatic endocrine cells co-expressing NKX6.1 and insulin. Method involves treatment of pancreatic endoderm cells obtained during human pluripotent stem cell differentiation, except for method, wherein human pluripotent stem cells are obtained by using a human embryo, a factor capable of inhibiting BMP, a TGFβ receptor signalling inhibitor and protein kinase C activator.
EFFECT: invention widens the range of equipment.
21 cl, 9 dwg, 4 tbl, 6 ex
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