FIELD: biotechnologies.
SUBSTANCE: invention relates to protection of plants. Disclosed is method of increasing efficiency of baculovirus preparations. Method involves infecting insects with nuclear polyhedral disease virus, for this purpose worms of I-III ages are fed during 2 days with nutrient medium containing 103-104 polyhedron per 1 mg of medium. In 2-5 days after infection external treatment of insect is performed using spray gun with aqueous solution of conservative short 18 nucleotides with length of gypsy moth nuclear polyhedral disease virus antisense fragment antiapoptotic gene of IAP-3 with sequence of 5′-CGACGTGGTGGCACGGCG-3′ in concentration of 10 pmol/mql.
EFFECT: invention provides faster action of baculovirus preparations.
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