BIVALENT BISPECIFIC ANTIBODIES Russian patent published in 2016 - IPC C07K16/46 

FIELD: medicine.

SUBSTANCE: invention relates to immunology and provides a method of producing a bivalent bispecific antibody, wherein said antibody contains a first light chain and a first heavy chain of an antibody specifically bound to a first antigen; and a second light chain and a second heavy chain of an antibody specifically bound to second antigen, in which variable domains VL and VH of second light chain and a second heavy chain are replaced by each other, where CH3 domain of one heavy chain and CH3 domain other heavy chain are in contact with each other by surfaces that are modified to activate formation of a bivalent bispecific antibody. Method involves following steps: transformation of host cell with expression vectors and host cell cultivation in conditions which enable synthesis of said bivalent bispecific antibody, and recovery of bivalent bispecific antibody from culture.

EFFECT: invention provides efficient production of bispecific antibodies to two antigens.

2 cl, 15 dwg, 4 ex