BISPECIFIC ANTIBODIES Russian patent published in 2016 - IPC C07K16/46 C07K16/22 C07K16/28 

FIELD: chemistry.

SUBSTANCE: invention relates to field of biotechnology and immunology and represents method for production of bispecific antibody, which contains: a) heavy chain and light chain of first full length antibody, which specifically binds with first antigen; and b) heavy chain and light chain of second full length antibody, which specifically binds with second antigen, where N-end of heavy chain is bound with C-end of light chain via peptide linker, with each of CH3-domain of heavy chain of full length antibody a) and CH3-domain of heavy chain of full length antibody b) being found on contact surface, which includes changes in initial contact surface of CH3-domains of antibody; wherein changes in CH3- domains of heavy chains represent changes of "key-lock" type; wherein said method includes the following stages: a) transformation of host-cell with vectors, containing nucleic acid molecules, coding said molecule of bispecific antibody; b) cultivation of host cell under conditions, providing synthesis of said molecule of bispecific antibody; and c) isolation of said molecule of bispecific antibody from said culture.

EFFECT: invention makes it possible to obtain bispecific antibodies with said structure with high output and purity.

17 cl, 10 dwg, 6 tbl, 11 ex