FIELD: biotechnologies.
SUBSTANCE: invention relates to biotechnology and virology. Described is method of purifying vectors based on recombinant adenoassociated virus (rAAV). Vectors based on recombinant AVV according to invention deprived of technological impurities, including components of product, such as cell nucleic acids, cell proteins, helper virus and components.
EFFECT: method involves bringing stream of initial materials in contact with medium for chromatography on apatite in presence of polyethylene glycol (PEG) and elution of rAAV particles using elution buffer containing less than 3 % (wt/Vol) PEG.
34 cl, 7 dwg, 6 tbl, 13 ex
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