FIELD: biotechnology.
SUBSTANCE: invention relates to a method for purification of an adeno-associated virus (hereinafter – AAV). The method for purification of AAV includes loading of a solution containing AAV to affine resin; at least two washing stages; and elution of AAV from affine resin. At the same time, at least one of washing stages includes application to affine resin of a buffer containing an organic solvent or a detergent. The method additionally includes bringing into contact of the solution containing AAV with anionite, and elution of the solution containing AAV from anionite before loading of the solution containing AAV to affine resin.
EFFECT: implementation of the invention provides an increase in the efficiency of purification of AAV.
80 cl, 21 dwg, 44 tbl, 18 ex
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