METHOD OF PURIFYING BACTERIOPHAGES Russian patent published in 2016 - IPC C07K14/01 C12N7/02 

FIELD: biotechnology.

SUBSTANCE: present invention relates to a method of purifying bacteriophages. Described method includes: a) culturing a bacterial host strain on an appropriate medium, inoculating said culture with a bacteriophage and obtaining a bacteriophage lysate; b) purifying lysate using affinity chromatography; c) using resulting filtrate to prepare a purified bacteriophage, wherein in stage a) bacterial host strain used consists of bacterial cells containing a sequence encoding a fusion protein containing a foreign polypeptide exhibiting an affinity for a chromatography resin used in stage b) and a polypeptide from among structural phage proteins of bacteriophage present in resulting lysate, and said polypeptide having affinity for chromatographic resin, selected from a group comprising His-tag and GST.

EFFECT: disclosed invention enables single-stage and simultaneously time-efficient purification of phage preparations for therapeutic purposes and ensures preservation of antibacterial activity of bacteriophage in case of displacement of bacteriophage from resin, and in case of proteolytic release.

5 cl, 10 dwg, 1 tbl, 1 ex