FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to a modified tamavidin, and can be used for detection of biotin associated substance. Obtaining modified biotin-binding protein based on tamavidin with sequence SEQ ID NO: 2, which contains up to 7 mutations and asparagine residue in 115 position is replaced with cysteine.
EFFECT: invention increases resistance of biotin-binding protein to heating and processing in dimethyl sulphoxide.
9 cl, 3 dwg, 3 tbl, 5 ex
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