FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology, specifically to production of a mutant protein associated with DNA replication (Rep), and can be used for diagnosing multiple sclerosis (MS). Synthesized single mutant C154E and double mutant 27/154E protein Rep are characterized by high stability and lower aggregation compared to the wild-type Rep protein in in vitro conditions, as well as in a storage buffer with urea.
EFFECT: invention enables producing more stable antigens than wild-type Rep MSBI1 and MSBI2 proteins for use in MS diagnostic screening assays.
10 cl, 6 dwg, 1 tbl, 4 ex
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