FIELD: biology.
SUBSTANCE: invention relates to molecular biology and can be used in biomedical research. Disclosed is a method of preparing a suspension of human lymphocytes in ethanol-acetic retainer for DNA recovery, which includes centrifugation of said suspension and removal of supernatant. Then obtained preparation is dried for 5 minutes at temperature of 55 °C, followed by adding physiological solution.
EFFECT: method ensures preservation of qualitative and quantitative characteristics of sample, acceptable for further polymerase chain reaction.
1 cl, 3 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR RECOVERY OF NUCLEIC ACIDS | 2014 |
|
RU2584346C2 |
| METHOD FOR DNA ISOLATION FROM PLANTS, SUITABLE FOR PCR | 2017 |
|
RU2672378C1 |
| METHOD OF DETERMINING LEVEL OF CHIMERIC GENE Trim5a EXPRESSION | 2015 |
|
RU2592675C1 |
| METHOD OF ISOLATION AND PURIFICATION OF DEOXYRIBONUCLEIC ACIDS | 2008 |
|
RU2400537C2 |
| TEST SYSTEM FOR DETECTING DNA OF CAUSATIVE AGENT OF MANNHEIMIOSIS (MANNHEIMIA HAEMOLYTICA) IN BIOLOGICAL MATERIAL OF ANIMALS AND FODDERS USING REAL-TIME POLYMERASE CHAIN REACTION | 2023 |
|
RU2814548C1 |
| METHOD FOR DETECTION OF BOVINE LEUKOSIS VIRUS (BLV) PROVIRUS DNA IN FOOD BY REAL-TIME POLYMERASE CHAIN REACTION | 2022 |
|
RU2794654C1 |
| METHOD FOR DETECTING DNA OF CHLOMYDIA IN FARM ANIMALS AND BIRDS | 2018 |
|
RU2700381C1 |
| TEST SYSTEM FOR IDENTIFYING THE DNA OF A CRYPTOSPORIDIOSIS PATHOGEN IN BIOLOGICAL MATERIAL OF ANIMALS AND IN FEEDS USING REAL-TIME POLYMERASE CHAIN REACTION | 2021 |
|
RU2785381C1 |
| TEST SYSTEM FOR GENOME DETECTION OF A CORONAVIRUS INFECTION AGENT OF NEW TYPE (NCOV19) IN PRIMATES | 2020 |
|
RU2741887C1 |
| METHOD FOR DETECTING THE DNA OF THE CAUSATIVE AGENT OF CRYPTOSPORIDIOSIS IN ANIMAL BIOLOGICAL MATERIAL AND FEED USING A POLYMERASE CHAIN REACTION IN REAL TIME | 2021 |
|
RU2782427C1 |
