TEST SYSTEM FOR GENOME DETECTION OF A CORONAVIRUS INFECTION AGENT OF NEW TYPE (NCOV19) IN PRIMATES Russian patent published in 2021 - IPC C12Q1/68 

FIELD: virology.

SUBSTANCE: invention refers to veterinary virology, namely to means of diagnosing coronavirus infection in primates. Described is a test system for detecting the novel genome of coronavirus infection (nCov19) in primates using a multiplexed polymerase chain reaction with real-time fluorescence detection, including a buffer for polymerase chain reaction. Test system is a mixture consisting of deoxynucleoside triphosphates, primers and fluorescent probes specific for coronavirus and for an internal control sample in form of suspension of bacteriophage MS2 with concentration of 5×10³ copies of nucleotide sequences per 1 mcl; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE and reverse transcriptase MMLV REVERSE TRANSCRIPTASE; a buffer for diluting RNA in the form of deionized water, an internal control sample, a negative control sample, positive control sample in form of a mixture of recombinant plasmid DNA containing a coronavirus infection genome fragment and a bacteriophage MS2 genome fragment taken in ratio 1:1, according to the invention, the mixture of recombinant plasmid DNA contains a fragment of the coronavirus infection genome of a new type (nCov19) and a fragment of the bacteriophage MS2 genome with the following nucleotide sequences: nCoV19-F 5’- GGAACTTCTCCTGCTAGA 3’ - forward primer, nCoV19-R 5’- GCTGGTTCAATCTGTCAA -3’ - reverse primer, nCoV19-P 5’-ROX- AAGCAAGAGCAGCATCACCGC -3’ -BHQ2 - probe, MS2F, 5’-TGGCACTACCCCTCTCCGTATTCAC-3’ - forward primer, MS2R, 5’-GTACGGGCGACCCCACGATGAC-3’ - reverse primer, MS2P, Cy5 5’-CACATCGATAGATCAAGGTGCCTACAAGC-3’-BHQ2 - probe.

EFFECT: invention broadens functional capabilities and reliability of diagnosis by RT-PCR in real time.

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