FIELD: medicine.
SUBSTANCE: method for cultivation of tylosis tissue Centaurea Scabiosa l. involves preparing a tylosis culture of intact plant explants by preparing the Murashige and Skoog nutrient medium to be dispensed for tylosis culture cultivation. The tylosis culture is prepared on the Murashige and Skoog medium with added 0.5-1 mg/l of α-naphthyl acetic acid and 0.2-0.5 mg/l of 6-benzylaminopurine; the Murashige and Skoog medium is despensed in plastic Petri dishes; the tylosis culture is transferred into said dishes that is followed by cultivation of the tylosis culture in the plastic Petri dish in blue light (380-560 nm).
EFFECT: invention provides higher growth index of the tylosis culture.
1 dwg, 1 tbl, 2 ex
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Authors
Dates
2012-08-10—Published
2011-05-11—Filed