FIELD: biotechnology. SUBSTANCE: invention relates to technology of growing transplanting cultures of vegetable tissues and can be used in medicinal industry for preparing new high-effective medicinal preparations. Agarized medium containing macrosalts and trace salts by Murasige-Skoog, vitamins and growth hormones is modified by hormonal composition 6- BAP and 2,4-D. Medium has additionally folic acid, riboflavin, biotin, pantothenate calcium and nicotine- -amide as the source of vitamin PP. Salts and vitamins used in proposed concentrations ensures to obtain callus tissue with culturing period 21 days and the yield of biomass 7.14-12.77 g/l. EFFECT: increased yield of biomass. 1 tbl
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Authors
Dates
2001-06-27—Published
1999-11-02—Filed