METHOD FOR QUANTITATIVE ESTIMATION OF BINDING FORCE OF SENSITISED POLYSTYRENE MICROSPHERES WITH EUCARYOCYTE LINE J-774 BASED ON OPTICAL TRAP METHOD Russian patent published in 2016 - IPC C12Q1/02 G01N33/53 A61B10/00 

FIELD: biochemistry.

SUBSTANCE: disclosed is a method for quantitative estimation of binding force of sensitised polystyrene microspheres with eucaryocyte line J-774. Method involves preparation of a culture adhered eucaryocytes J-774, calibration optical forceps, using a piezo table to feed J-774 cell to held microsphere until contact thereof, removal of piezo table with automatic detection of dynamics of interaction between cell and microsphere. Precision feeding of cell to microsphere is carried out in manual mode with step bringing. Moment of contact of microscopic objects established based on presence on continuously recorded force curve of changes, corresponding values of reliability of contact. Contact reliability indicators include emergence on a chronogram signal of three inverted steps of high amplitude in response to three consecutive steps of table, as well as preceding said indicators variation of amplitude of Brownian displacements of microspheres. Force of breakoff of microspheres from cell is automatically calculated from amplitude of characteristic jump on force curve.

EFFECT: invention provides improved control of quantitative estimation of binding force of sensitised microspheres and fixed macrophages, high accuracy of changing parameters describing said force.

1 cl, 2 dwg, 3 ex