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IPC

C12N1/21(2006-01-01)

C12N9/42(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2016125816, 2016-06-28

(24)

Start date

2016-06-28

(22)

Actual filing date

2016-06-28

(45)

Published

2017-07-11

(72)

Inventor

Rykov Sergej ViktorovichBerezina Oksana ValentinovnaSakhibgaraeva Liliya FlyuzovnaKrestyanova Irina NikolaevnaYarotskij Sergej Viktorovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauchno-Issledovatelskij Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov"

RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF XYLOGLUCANASE FROM ASPERGILLUS CERVINUS FUNGI AND METHOD FOR XYLOGLUCANASE MICROBIOLOGICAL SYNTHESIS BASED ON THIS STRAIN Russian patent published in 2017 - IPC C12N1/21 C12N9/42 C12R1/19

Abstract RU 2625013 C1

FIELD: biotechnology.

SUBSTANCE: recombinant strain of Escherichia coli VKPM B-12683, a producer of GH12 family xyloglucanase encoded by the AsCeGH12b (SEQ ID NO 1) gene cloned from Aspergillus cervinus VKPM F-612 is proposed. The strain of Escherichia coli VKPM B-12683 was obtained by Escherichia coli BL21-CODONPLUS (DE3)-R1PL strain transformation with pET-AsCeGH12b (SEQ ID NO 3) plasmid. A method for microbial synthesis of GH12 family xyloglucanase encoded by the AsCeGH12b gene cloned from Aspergillus cervinus VKPM F-612 by Escherichia coli VKPM B-12683 strain culturing under aerobic conditions in a suitable nutrient medium to maximize the accumulation of the desired product is proposed.

EFFECT: high level of target product biosynthesis.

2 cl, 1 dwg, 4 ex

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RU 2 625 013 C1

Authors

Rykov Sergej Viktorovich

Berezina Oksana Valentinovna

Sakhibgaraeva Liliya Flyuzovna

Krestyanova Irina Nikolaevna

Yarotskij Sergej Viktorovich

Dates

2017-07-11—Published

2016-06-28—Filed