MUTANT FORM OF XYLOGLUCANASE FROM ASPERGILLUS CERVINUS WITH INCREASED THERMAL STABILITY COMPARED TO WILD-TYPE ENZYME, RECOMBINANT ESCHERICHIA COLI STRAIN PRODUCING THIS XYLOGLUCANASE, AND MICROBIOLOGICAL METHOD SYNTHESIS OF MUTANT XYLOGLUCANASE FROM ASPERGILLUS CERVINUS BASED ON THIS STRAIN Russian patent published in 2024 - IPC C12N9/24 C12N1/21 C12N15/56 C12N15/70 

FIELD: biotechnology.

SUBSTANCE: mutant xyloglucanase AsCeGH12b-mut having the amino acid sequence SEQ ID NO 1, which corresponds to the amino acid sequence of wild-type family 12 xyloglucanase from Aspergillus cervinus VKPM F-612, with the replacement of the natural tyrosine residue at position 37 with a valine residue, and increased thermal stability and temperature maximum activity compared to the wild type enzyme is proposed. Also a recombinant strain of Escherichia coli VKPM B-14321, producing the specified xyloglucanase AsCeGH12b-mut, and a method of microbiological synthesis of the specified xyloglucanase using the specified strain are proposed.

EFFECT: expansion of the range of recombinant xyloglucanases, producer strains and methods of microbiological synthesis of recombinant xyloglucanases.

3 cl, 2 dwg, 3 ex