FIELD: biotechnology.
SUBSTANCE: inventions group relates to biotechnology. A recombinant yeast strain Komagataella phaffii RNCIM Y-4933 is proposed, producing a secreted endo-dissociative xyloglucanase of the GH74 family, encoded by a mutant gene corresponding to SEQ ID NO 1. A method for microbiological synthesis of xyloglucanase using the specified strain is also proposed.
EFFECT: inventions group provides a high level of production of xyloglucanase of the GH74 family with an endo-dissociative mode of action on the substrate, providing an effective decrease in the viscosity of xyloglucan solutions.
2 cl, 3 ex, 2 dwg
