FIELD: medicine.
SUBSTANCE: invention refers to medicine and concerns a method of producing a medicinal agent based on human vesicles for stimulation of angiogenesis in patients with ischemic tissue damage, where human cells of line SH-SY5Y are grown on culture bottle, washed by isotonic buffer solution, sterile solution of cytochalasin B added to the cells, cells are washed with isotonic buffer solution, transferred into suspension, subjected to active mixing and a series of centrifugations carried out to produce the declared drug.
EFFECT: invention provides preparing of the therapeutic agent, having increased in comparison with the prototype and analogues therapeutic action and angiogenic potential, as well as meets criterion of the biological safety.
1 cl, 1 ex, 5 dwg
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Authors
Dates
2016-12-27—Published
2015-07-27—Filed