FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used in pharmaceutical and food industry. Method comprises bacterial transformation of explant root of juvenile plant Silene linicola by agro bacterial strain R-1601 A. Rhizogenes. Transformed roots of explant is separated and cultured. Isolated roots cultured first two passages on Gamborg medium with addition of 250 mg/l cefotaxime, subsequent passages are grown on a Gamborg medium with addition of 500 mg/l casein hydrolysate.
EFFECT: invention allows to ensure permanent high rate of growth of isolated roots of Silene linicola K1601 on non hormonal culture media at high level of biosynthesis of species-specific ecdysteroids.
1 cl, 3 dwg, 2 tbl, 2 ex
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