FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology. Method of producing capsular polysaccharide with pneumococcal serotype is disclosed. Bacterial cells are cultured at culture fluid pH in range of 7.0 to 9.4. Then cultivation is stopped at the moment between culture fluid absorption coefficient is constant and absorption coefficient begins to fall. Additional cultivation is performed without pH regulation until culture fluid pH reaches value of 5.5 or less. Then lysing agent is added to obtained culture fluid for cell lysis. Precipitated proteins and cellular debris are removed to obtain purified cell lysate. Capsular polysaccharide is separated and purified from obtained lysate.
EFFECT: invention eliminates process of depositing protein by increasing acidity by means of pH regulator, which simplifies process and does not lead to modification of capsular polysaccharide and formation of hazardous substances.
13 cl, 6 dwg, 10 tbl, 3 ex
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