METHOD OF MODIFICATION OF POLYPEPTIDE FOR PURIFICATION OF POLYPEPTIDE MULTIMERS Russian patent published in 2017 - IPC C12N15/00 A61K39/395 C07K1/22 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and represents method of polypeptide multimer production, which includes first polypeptide with antigen-binding activity and second polypeptide with antigen-binding activity or in addition to first and second polypeptides multimer includes one or two third polypeptides with antigen-binding activity or in addition to first, second and third polypeptides multimer includes fourth polypeptide with antigen-binding activity; and method involves following steps: (a) expression of DNA, which codes first polypeptide with antigen-binding activity, and of DNA, which codes second polypeptide with antigen-binding activity, or expression of DNA, which codes first, second and third polypeptides with antigen-binding activity, or expression of DNA, which codes first, second, third and fourth polypeptides with antigen-binding activity; and (b) assembling of expression product of stage (a) using affinity chromatography with protein A.

EFFECT: invention enables to obtain multimers with antigen-binding activity of high purity as a result of only purification step, based on protein A.

44 cl, 18 dwg, 18 tbl, 12 ex