FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents method of polypeptide multimer production, which includes first polypeptide with antigen-binding activity and second polypeptide with antigen-binding activity or in addition to first and second polypeptides multimer includes one or two third polypeptides with antigen-binding activity or in addition to first, second and third polypeptides multimer includes fourth polypeptide with antigen-binding activity; and method involves following steps: (a) expression of DNA, which codes first polypeptide with antigen-binding activity, and of DNA, which codes second polypeptide with antigen-binding activity, or expression of DNA, which codes first, second and third polypeptides with antigen-binding activity, or expression of DNA, which codes first, second, third and fourth polypeptides with antigen-binding activity; and (b) assembling of expression product of stage (a) using affinity chromatography with protein A.
EFFECT: invention enables to obtain multimers with antigen-binding activity of high purity as a result of only purification step, based on protein A.
44 cl, 18 dwg, 18 tbl, 12 ex
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Authors
Dates
2017-01-10—Published
2010-12-24—Filed