FIELD: medicine.
SUBSTANCE: present invention relates to immunology. Proposed is a method for obtaining a bispecific antibody that involves the introduction of amino acid residues that electrically repel one another to position 147 of heavy chain and 180 of light chain, 147 of heavy chain and 131 of light chain or 175 of heavy chain and 160 of light chain (according to EU numbering), where the heavy chain is IgG1, and the light chain is IgK (Kappa). Also a method is proposed for regulating the association of a heavy chain and a light chain of a bispecific antibody in such a way that no undesirable associations form between the heavy and light chains.
EFFECT: invention suppresses any undesirable association between the heavy chain constant region 1 (CH1) and the constant region of the light chain (CL) by replacing the amino acids that are present at the border between CH1 and CL with electrically charged amino acids.
12 cl, 12 dwg, 14 tbl, 9 ex
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