METHOD OF PRODUCING MODIFIED ALGINATE MICROSPHERES Russian patent published in 2017 - IPC A61K9/14 A61K35/12 A61K35/407 A61K47/36 A61K47/48 

FIELD: biotechnology.

SUBSTANCE: invention relates to investigation of biomaterials and tissue engineering, namely, to a method of producing modified alginate microspheres, involving liver decellularization, its mechanical slicing and further mechanical milling to produce microparticles of decellularized liver, that is fixed by covalent binding on preliminary prepared alginate microspheres, modified using modifying agents, such as hydroxysuccinimide (NHS) and hydrochloride N-(3-dimethylaminopropyl)-N-ethylcarbodiimide (EDC). Invention provides high density of microparticles of decellularized hepatic tissue on the surface of modified alginate microcarriers and a strong covalent bond between them.

EFFECT: proposed modified alginate microspheres can act as biodegradable container for biologically active substances and/or cells, as well as to protect the cells, encapsulated inside alginate microcarriers from act of recipient's immune system when implanted.

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