TREATMENT METHOD OF LIVE FAILURE Russian patent published in 2017 - IPC A61B17/00 A61M37/00 A61K38/39 A61K35/28 A61K35/407 A61K8/65 A61K33/44 A61K33/14 A61P1/16 

FIELD: medicine.

SUBSTANCE: invention refers to the medicine, cellular transplantology, and hepatology. An implantation of a cell-engineering structure (CES) is performed into the liver parenchyma with the further prescription of anticoagulants and antiaggregants of a preventive dose. Whereupon, first, a matrix from a decellularized donor liver of a mammal in the physiological solution buffered with phosphates and having the following composition: 138 mM NaCl, 2.67 mM KCl, 1.47 mM KH₂PO₄, 8.1 mM Na₂HPO₄, distilled water under 1 l and containing fibronectin and laminin in the amount of 10 µg/ml, with pH 7.4, is incubated during 8-12 hours at 4°C. The volume ratio of the matrix and physiological solution is 1:1. Then, a co-culture of the freshly-isolated allogenic liver cells and preliminarily cultured allogenic or autologous mesenchymal stem cells of the bone marrow with the ratio of the bone marrow cells to the liver cells from 1:1 to 1:10 is conducted on the matrix during 2-4 days providing the attachment of the cells in the amount of 210⁶-1510⁶ per 1 cm³ of the matrix. The total matrix volume with the attached cells is at least 0.05 cm³. The CES is obtained before the transplantation by mixing the matrix and the cells attached to it with the biopolymer heterogenic collagen-containing hydrogel with the volume ratio of 3:1. In a separate case, the matrix is obtained from a decellularized donor liver of a human. The matrix may have particle sizes from 200 to 700 mcµm, pore sizes of no more than 50 mcm, and a summarized porosity of 70-85%. Spherogel may be used as a heterogenic biocompatible biodegradable gel.

EFFECT: method of improving of the treatment of the liver failure by means of actuation of bilateral interactions between the implanted CESs as a ready hepatolike structures and the parenchyma of a damaged liver of a recipient with the quickened integration of them into the damaged liver.

4 cl, 5 dwg