METHOD OF PRODUCING XENOGRAFT FOR OPHTHALMOSURGERY Russian patent published in 2017 - IPC A61F2/00 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, particularly ophthalmology, and can be used for producing graft-collagen material to perform sclero-plastic surgical interventions in treating progressive myopia of medium and high degree. Method comprises mechanical cleaning, treatment with 10 % ammonia solution for 4 hours, washing with purified water, multiple freezing and thawing of biomaterial, treatment with 6 % hydrogen peroxide solution, biomaterial fragmentation, dehydration in alcohols of rising concentration from 30 to 70 vol%, packing in bottles with 70 % ethyl alcohol and sterilisation with ionising radiation dose of 1.5 Mrad. In addition, two-step treatment with 15 % formic acid, first, after first freezing and thawing for 1.5 hours while stirring, then, after repeated freezing and thawing for 1 hour while stirring. Also, before dehydration method includes further ultraviolet cross-linking of collagen biomaterial by its treatment with 0.5 % riboflavin solution for 20 minutes and subsequent ultraviolet irradiation with power 5 mW/cm² at wavelength 370 nm for 20 minutes.

EFFECT: using the invention increases strength and mechanical characteristics and biocompatibility xenograft.

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