METHODS OF PRODUCING VIRAL PARTICLES WITH SIMPLIFIED SURFACE PROTEINS' GLYCOSYLATION Russian patent published in 2017 - IPC C12N7/00 C12P21/00 C07K14/11 A61P31/16 

Abstract RU 2607452 C2

FIELD: biotechnology.

SUBSTANCE: present invention relates to biotechnology. Method for production of influenza virus with monoglycosylated hemagglutinin-antigen (HA-antigen) is disclosed. Method involves cultivation of influenza virus, containing hemagglutinin-antigen in specific pathogen free (SPF) chicken egg with embryo with an effective amount of mannosidase inhibitor, concentration of which is sufficient for inhibiting of α-mannosidase I in the path of N-glycosylation followed by extraction of produced influenza virus. Further contact of extracted influenza virus with endoglycosidase (EndoH) leads to production of influenza virus, having monoglycosylated influenza virus HA-antigen.

EFFECT: disclosed method enables to obtain influenza virus with monoglycosylated hemagglutinin-antigen (HA-antigen) with high yield using specific pathogen free (SPF) chicken eggs with embryo, and can be used for producing monoglycosylated hemagglutinin-antigen in production of vaccines.

19 cl, 4 dwg, 4 ex

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RU 2 607 452 C2

Authors

Von Chi-Khuej

Ma Che

Tsen Yun-Chie

Dates

2017-01-10Published

2011-11-04Filed