FIELD: biotechnology.
SUBSTANCE: described is a chimeric hemagglutinin (HA) polypeptide of an influenza virus, used as an immunogen, comprising one or more stem domain sequences, each of which has at least 60% homology with the consensus sequence of the stem domain of the HA subtype (H1 HA) and/or HA subtype H5 (H5 HA), fused with one or more globular head domain sequences, each of which has at least 60% homology with the consensus sequence of the domain of the globular head of subtype H1 (H1 HA) or HA subtype H5 (H5 HA), where the chimeric hemagglutinin (HA) polypeptide of the influenza virus contains the amino acid sequence SEQ ID NO: 4, SEQ ID NO: 8 or SEQ ID NO: 12. Also described is an immunogenic composition containing a chimeric polypeptide of influenza virus. Disclosed is a method of immunizing a subject against an influenza virus or preventing a disease caused by an influenza virus, comprising administering to the subject an effective amount of a chimeric polypeptide of influenza virus (HA) according to any one of claims 1, 2 or immunogenic composition according to cl. 3, where the method increases production of IFN-gamma, IL-4 and CD8+ memory T-cells. Also disclosed is a recombinant polynucleotide encoding a polypeptide. Disclosed is an expression vector containing a recombinant polynucleotide, and a host cell containing the vector.
EFFECT: chimeric influenza vaccines are disclosed.
11 cl, 11 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
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| VACCINE TO SWINE INFLUENZA A VIRUS | 2018 |
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| RECOMBINANT VECTORS EXPRESSING AVIAN INFLUENZA VIRUS ANTIGENS AND APPLICATIONS THEREOF | 2017 |
|
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| VACCINE COMPOSITIONS CHARACTERIZED BY IMPROVED STABILITY AND IMMUNOGENICITY | 2016 |
|
RU2730625C2 |
| ANTIBODIES SUITABLE FOR PASSIVE IMMUNIZATION AGAINST INFLUENZA, AND COMPOSITIONS THEREOF, COMBINATIONS AND METHODS OF USING | 2015 |
|
RU2720282C1 |
| MATERIALS AND METHODS USED FOR TREATMENT OF RESPIRATORY DISEASES IN DOGS | 2020 |
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| ADVANCED CORONAVIRUS VACCINE | 2022 |
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RU2816182C2 |
