FIELD: biotechnology.
SUBSTANCE: method for detection of structural rearrangements of the tumor cells (chimeric genes) genome determining the selection of therapy and prediction in acute leukosis in children, using RT-PCR with subsequent hybridization with an oligonucleotide biological microchip (biochip). Detection of these translocations in the bone marrow tumor cells is the standard of modern diagnosis of leukemia and is used in most modern protocols for patients division to risk groups and selection of therapy, including high-dose cytotoxic drug therapy and/or bone marrow transplantation.
EFFECT: invention allows to identify 22 known chimeric genes.
3 cl, 2 dwg, 3 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DIAGNOSING ACUTE LEUKEMIA AND KITS FOR ITS IMPLEMENTATION | 2021 |
|
RU2806591C2 |
| METHOD FOR IDENTIFYING CHROMOSOMAL TRANSLOCATIONS LEADING TO THE DEVELOPMENT OF MALIGNANT BLOOD DISEASES (LEUKOSES), DUE TO APPLYING OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2004 |
|
RU2286798C2 |
| METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN THE BCR/ABL CHIMERIC GENE USING RT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2015 |
|
RU2609641C1 |
| ANALYSIS METHOD OF EML4-ALK TRANSLOCATIONS ASSOCIATED WITH SENSITIVITY OF LUNG CANCER TO ANTITUMOUR TARGET THERAPY | 2012 |
|
RU2509153C1 |
| METHOD OF POLYMORPHIC MARKERS ANALYSIS IN THE GENES OF METABOLISM OF MEDICINAL PREPARATIONS AND THE GENES OF IMMUNE RESPONSE IN THE ACUTE LEUCOSISES THERAPY IN CHILDREN | 2016 |
|
RU2643333C1 |
| DETECTION OF MOLECULAR COLONIES BY MEANS OF HYBRIDISATION ON MEMBRANES WITH FLUORESCENT PROBES | 2006 |
|
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| METHOD OF PRODUCING PANEL OF DNA PROBES FOR DETERMINING CHROMOSOMAL TRANSLOCATIONS, DELETIONS AND AMPLIFICATIONS | 2023 |
|
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| METHOD OF ANALYZING POLYMORPHIC MARKERS IN THE GENES VKORC1, CYP4F2, CYP2C9, CYP2C19, ABCB1, ITGB3 TO DETERMINE INDIVIDUAL SENSITIVITY TO ANTICOAGULANT DRUGS | 2018 |
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| METHOD FOR DETERMINING POLYMORPHIC MARKERS IN SLCO1B1, APOE AND ABCB1 GENES FOR DETERMINING INDIVIDUAL SENSITIVITY TO STATINS | 2018 |
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|
RU2716589C1 |
