METHOD OF PURIFYING FILAMENTOUS BACTERIOPHAGE M13 Russian patent published in 2017 - IPC C12N7/02 

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of purifying filamentous bacteriophage M13. Method involves removal of bacterial mass after producing bacteriophage by first and second successive centrifugation followed by first purification of supernatant of bacteriophage by isoelectric precipitation with a reagent at pH 4.2, further third centrifuging and re-suspension of precipitate with bacteriophage, as well as repeated purification of suspension of bacteriophage by isoelectric precipitation with a reagent at pH 4.2 and fourth centrifugation of precipitate with bacteriophage to produce end product. For isoelectric precipitation agent used is sodium acetate buffer with pH = 4.2 in ratio to supernatant of bacteriophages of not less than 3:1 at first isoelectric precipitation, precipitate with bacteriophage after third centrifugation is re-suspended in TBS-buffer, and during repeated purification of re-suspended bacteriophage by isoelectric precipitation, sodium acetate buffer with pH = 4.2 is used in ratio to re-suspended bacteriophage of not less than 1:1. First and second centrifugation is carried out at 5,000 rpm and 12,000 rpm for 10 minutes at 20 °C, and third and fourth centrifugation is carried out at 10,000 rpm for 10 minutes at 4 °C.

EFFECT: invention provides a higher level of sterility and purity of bacteriophage, especially in small quantities thereof using a simpler technique.

1 cl, 7 tbl, 9 ex