FIELD: biotechnology.
SUBSTANCE: method includes selection of an area of the chromosome with a DNA-marking section; obtaining of a capture probe corresponding to the DNA sequence in this area; hybridizing of the capture probe with a mixed library for binding to the sequence consisting of multiple DNA samples from the area with the DNA-marking section; sequencing of the captured DNA sequence from the area with the DNA-marking section in order to obtain sequencing data and sequencing results analysis using the method of mathematical statistics to obtain a result indicating the presence or absence of chromosome microdeletions in DNA-marking section area in each of the multiple DNA samples.
EFFECT: invention provides effective and accurate detection of known and unknown microdeletions in a chromosome with aDNA-marking section during processing of a large number of DNA samples.
14 cl, 29 dwg, 8 tbl
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| METHOD AND SYSTEM FOR DETECTING VARIATION OF NUMBER OF COPIES IN GENOME | 2012 |
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| NON-INVASIVE DETECTION OF GENETIC FOETAL ABNORMALITY | 2011 |
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| METHOD FOR NON-INVASIVE PRENATAL SCREENING OF FETAL ANEUPLOIDY | 2019 |
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| METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING | 2011 |
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| NON-INVASIVE DIAGNOSTIC TECHNIQUE FOR FOETAL ANEUPLOIDY BY SEQUENCE ANALYSIS | 2014 |
|
RU2543155C1 |
| METHODS OF SEQUENCING OF THREE-DIMENSIONAL STRUCTURE OF THE ANALYZED GENOME REGION | 2011 |
|
RU2603082C2 |
| SUPPRESSING ERRORS IN SEQUENCED DNA FRAGMENTS BY USING EXCESSIVE READING WITH UNIQUE MOLECULAR INDICES (UMI) | 2016 |
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