FIELD: biotechnologies.
SUBSTANCE: group of inventions relates to biotechnology. Proposed are a method and a system for determining nucleotide sequence in a given region of a foetus genome. Method comprises the following steps: constructing a library for sequencing from genomic DNA sample from peripheral blood of a pregnant woman; sequencing the library for sequencing in order to obtain a sequencing result. Result of the foetus sequencing includes multiple data obtained during sequencing. Nucleotide sequence in a given region of the genome is determined by using hidden Markov model basing on the result of sequencing the foetus sequences in combination with genetic information upon a relative using Viterbi algorithm. System consists of a device of the library constructing, a sequenator and an analyzer.
EFFECT: using the inventions enables performing a genetic typing of high accuracy.
14 cl, 2 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS AND SYSTEMS FOR DETERMINING WHETHER GENOME IS ABNORMAL | 2011 |
|
RU2599419C2 |
| DETECTION OF MUTATIONS AND PLOIDY IN CHROMOSOMAL SEGMENTS | 2015 |
|
RU2717641C2 |
| HIGHLY MULTIPLEX PCR METHODS AND COMPOSITIONS | 2012 |
|
RU2650790C2 |
| METHODS OF NONINVASIVE PRENATAL PATERNITY DETERMINATION | 2011 |
|
RU2620959C2 |
| METHOD FOR MICRODELETION DETECTION NEAR CHROMOSOME WITH DNA-MARKING SECTION | 2012 |
|
RU2610691C2 |
| METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING | 2011 |
|
RU2671980C2 |
| BIOINFORMATIC SYSTEMS, DEVICES AND METHODS FOR PERFORMING SECONDARY AND/OR TERTIARY PROCESSING | 2017 |
|
RU2750706C2 |
| BIOINFORMATION SYSTEMS, DEVICES AND METHODS FOR SECONDARY AND/OR TERTIARY PROCESSING | 2017 |
|
RU2799750C2 |
| METHODS OF SEQUENCING OF THREE-DIMENSIONAL STRUCTURE OF THE ANALYZED GENOME REGION | 2011 |
|
RU2603082C2 |
| MOLECULAR MARKERS ASSOCIATED WITH SUNFLOWER RESISTANCE TO OROBANCHE | 2018 |
|
RU2776361C2 |
