HIGH-EFFICIENCY SCREENING COMPOUNDS MODULATING EXPRESSION OF CELL MACROMOLECULES Russian patent published in 2017 - IPC G01N33/50 G01N33/52 

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine, namely to diagnostics. Method of identification and quantitative determination of specific target molecule in sample, involving: testing and selecting first ligand or multiple first and second ligands or multiple second ligands connected to detected mark, in terms of binding target-molecule; or selecting first ligand or multiple first and second ligands or multiple second ligands connected to detected mark, in terms of binding target-molecule; screening sample containing specific target-molecule in effective screening analysis, addition of first and second ligands, each of which has first and second detected mark, binding of each of first and second ligands with separate and specific sites on specific target-molecule, where screening analysis does not require washing step; detection of light emission, when first and second ligands are specifically bound with specific target-molecule; measurement of intensity of emitted light and light intensity is identification and quantitative determination of specific target-molecules in sample. Method of quantitative determination of specific protein in sample. Method for screening of potentially therapeutic compounds. Method for screening of potentially therapeutic compounds. Method of diagnosing disease or disorder.

EFFECT: above group of solutions enables identifying and determining number of specific target-molecules in sample.

32 cl, 19 dwg, 1 ex